ABSTRACT
Background: Life expectancy was one indicator of human development index in the health sector, one of which was affected by the telomere shortening process. There were many factors that cause shortening of telomere, including an imbalance of carbohydrate consumption then oxidative stress. The study aimed to examine the association between carbohydrate and carbohydrate simplex (sucrose) consumption with telomere length based on plasma malondialdehyde in Minangkabau male.Methods: The study was cross-sectional with 97 samples from male civil servant, Minangkabau ethnic, 40-50 years old in Padang City. Carbohydrate and sucrose consumptions were obtained through semiquantitative food frequency questionnaire, plasma malondialdehyde examination with thiobarbituric acid test and telomere length measured by quantitative polymerase chain reaction using O'Challagan and Fenech method.Results: The result showed mean of telomere length was 550.43±188.47 bp; mean of carbohydrate consumption 1280.97±433.57 kcal or 48.80±16.52%; mean of sucrose consumption 179.40±126.61 kcal or 6.83±4.82% and mean plasma malondialdehyde 66.91±13.93 nmol/ml. The association of carbohydrate consumption with telomere length based on plasma malondialdehyde was obtained p-value = 0.714, 0.908 and 0.903. The relationship of sucrose consumption with telomere length based on malondialdehyde was obtained p-value=0,667 and 1,000. Differences in mean telomere length in various categories of carbohydrate and sucrose consumption obtained p-value = 0.547 and 0.559.Conclusions: There was no significant association between carbohydrate and carbohydrate simplex (sucrose) consumption with telomere length based on plasma malondialdehyde of Minangkabau male; and no significant difference in telomere length in different categories of carbohydrate consumption and different sucrose consumption.
ABSTRACT
Background: Non-communicable diseases (NCD) are the leading cause of death globally today. In West Sumatra there has been an increase in NCD which exceeds the national average percentage. Telomere shortening is associated with occurrence of NCD. Shortening of telomeres is influenced by diet. The purpose of this study was to examine the relationship between Saturated Fatty Acid (SAFA) intake and Superoxide Dismutase (SOD) activity with the telomere length of Minangkabau ethnic men.Methods: This study was a cross sectional study. The sample of this study were 69 civil servants of Minangkabau male at district office in the Padang City, aged 40-50 years and according to the inclusion and exclusion criteria. Data on SAFA intake was obtained using Semi Quantitative Food Frequency Questionnaire (SQFFQ), SOD activity was measured using the Calorimetric method and telomere length was measured using Real Time Polymerase Chain Reaction (RT-PCR) by O’Challaghan and Fennech method. Data analysis was performed using Pearson correlation test with significance level p <0.05.Results: The results showed an average SAFA intake 10.47+3.46% calories, SOD activity 5.93+0.81units/ml and telomere length 468.94+199.25bp. Correlation test between telomere length with SAFA intake showed (p=0.338), and the correlation test between SOD activity and telomere length showed (p=0.01).Conclusions: From this study it was concluded that there was no correlation between SAFA intake and telomere length and there was a significant positive correlation between SOD activity and the telomere length of Minangkabau ethnic men.
ABSTRACT
Background: Excessive carbohydrate and fat intake with excess estradiol levels will cause an increase in body fat percentage, thus causing obesity in premenopausal women. Objective was to determine the association between carbohydrate intake with fat percentage, the association between fat intake with fat percentage, the association between SAFA intake with fat percentage, the association between MUFA intake with fat percentage, the association between PUFA intake with fat percentage, and the association between estradiol levels with fat percentage in Minangkabau ethnic premenopausal women in Padang City, West Sumatra.Methods: This research was carried out in Padang City. Research conducted was observational research with cross sectional research design. Subjects in this research were 52 people. Variables in body fat percentage were measured using Bioelectrical Impedance Analysis (BIA), carbohydrate and fat intake data were obtained by interviewing the Food Frequency Questionnaire (FFQ), and estradiol levels were examined by ELISA. Data analysis was performed using Pearson correlation test.Results: In premenopausal women in the city of Padang, low carbohydrate intake was found with an average of 227.25grams, high fat intake was found with an average of 77.84grams, high SAFA intake was found with an average of 27.59grams, low MUFA intake was found with an average of 9.91grams, normal PUFA intake was found with an average of 9.37grams, normal estradiol levels was found with an average of 145.95pg/ml, and more fat percentage was found with an average of 33.31.Conclusions: There was an association between carbohydrate intake with fat percentage, there was an association between fat intake with fat percentage, there was an association between SAFA intake with fat percentage, there was an association between MUFA intake with fat percentage, there was an association between PUFA intake with fat percentage, and no association between estradiol levels with of fat percentage in premenopausal women in Padang City.
ABSTRACT
Background: Hyperuricemia is a predictor of metabolic syndrome influenced by many factors, one of which is nutritional status. A highly prevalence overweight and obesity in ethnic Minangkabau is quite high which can increase the prevalence of hyperuricemia. This study aims to determine the relationship of nutritional status intake with Minangkabau ethnic male uric acid levels.Methods: This study used a cross-sectional design, done in August to November 2016. Nutritional status was obtained through the measurement of body mass index (BMI) based on height and weight. The uric acid level examined in the Prodia laboratory. Data were analyzed by using chi-square test.Results: Most of subject were obesities. A total of 21% subjects suffers hyperuricemia. Statistic test results showed that there was a significant relationship between nutritional status and serum uric acid levels (p <0.0001).Conclusions: There was a significant relationship between nutritional status and serum uric acid levels in Minangkabau ethnic men in Padang city.